在建立‘藤稔’等５个葡萄品种离体培养快速繁殖体系的基础上，通过热处理和微茎尖脱毒培养获得了再生植株；并经过ＰＡＳ－ＥＬＩＳＡ和长臂光敏生物素标记ＧＦＬＶ－ＣＰ基因ｃＤＮＡ探针检测．结果表明白天３８℃夜晚３５℃处理脱除ＧＦＬＶ效果最佳；多次继代培养也有脱除ＧＦＬＶ的作用．从而首次获得了‘藤稔’、‘红地球’、‘９３０７’３个葡萄品种的脱除ＧＦＬＶ的试管苗．ＰＡＳ－ＥＬＩＳＡ与探针平行检测的结果还表明，ＰＡＳ－ＥＬＩＳＡ检测以Ｐ／Ｎ＜１作为阴性标准更为可靠 The regenerated plants were obtained through heat treatment and detoxification of the apical meristem based on the establishment of the rapid propagation system of five grape cultivars such as ’Fujiminori’. In addition, PAS-ELISA and long-arm biotin-labeled GFLV-CP Gene cDNA probe detection. The results showed that the removal of GFLV was best at 35 ℃ during the daytime at 38 ℃, and the removal of GFLV was also observed in multiple subcultures. As a result, GFLV-depleted plantlets were obtained for the first time in ’Fujimoto’, ’Red Globe’ and ’9307’3 grape varieties. The results of PAS-ELISA in parallel with the probe also showed that the PAS-ELISA assay was more reliable with a P / N <1 as the negative standard