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目的了解成都市婴幼儿腹泻患者人博卡病毒检出率、亚型分布以及VP1基因变异特征。方法收集四川大学华西第二医院2010年4月-2012年9月期间儿童腹泻感染病例标本共计749例,运用PCR方法检测HBoV VP1 DNA,随机选取25例阳性标本进行VP1基因测序,构建进化树并比较其与基因文库参考序列之间的差异。结果 HBoV的检出率为25.77%(193/749),随机选取的25例阳性标本中,检出HBoV2和HBoV3各1例,其余23例为HBoV1。各亚型阳性标本VP1基因核苷酸序列与参考序列相比均没有明显差异,其中HBoV1亚型VP1基因与其参考序列相似性大于99%,HBoV2为97.5%,HBoV3为98.6%。结论成都地区婴幼儿腹泻患者博卡病毒检出率较高,以HBoV1亚型为主。
Objective To understand the prevalence and distribution of human Boca in infants with diarrhea in Chengdu and the variation of VP1 gene. Methods A total of 749 cases of childhood diarrhea were collected from April 2010 to September 2012 in Huaxi Second Hospital of Sichuan University. VP1 DNA was detected by PCR and 25 cases of positive samples were selected randomly to construct phylogenetic tree Compare the difference with the gene library reference sequence. Results The positive rate of HBoV was 25.77% (193/749). Of the 25 positive samples randomly selected, one was found for HBoV2 and one for HBoV3, the other 23 were HBoV1. There was no significant difference in the nucleotide sequence of VP1 gene between the subtype positive samples and the reference sequence. The VP1 gene of HBoV1 subtype was more than 99% identical to its reference sequence, HBoV2 was 97.5% and HBoV3 was 98.6%. Conclusions The detection rate of Bocavirus in infants and children with diarrhea in Chengdu is higher than that in HBoV1 subtype.