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通过制备量子点荧光检测探针,构建了一种基于量子点探针和免疫磁珠的蛋白质检测方法,实现了对蛋白肿瘤标志物癌胚抗原(carcinoembryonic antigen,CEA)的高灵敏定量检测.在该检测体系中,若存在有靶标蛋白,其与量子点检测探针以及捕获探针之间会发生免疫反应形成三明治结构,利用磁力分离器对免疫复合物进行富集后,通过检测富集在磁珠表面的量子点荧光信号,可实现对靶标蛋白的定量.该方法的检测灵敏度为38 pg/mL,线性范围为0.39~50ng/mL,临床质控样本检验结果表明,该方法准确度高,可重复性好,可应用于临床样本检测.该量子点探针检测体系具有灵敏度高、特异性好、样品消耗量低等优点,在疾病早期诊断方面具有广阔的应用前景.
Through the preparation of quantum dot fluorescence detection probe, a protein detection method based on quantum dot probes and immunomagnetic beads was constructed to achieve highly sensitive and quantitative detection of carcinoembryonic antigen (CEA) In the detection system, if there is a target protein, it will have an immunological reaction with the quantum dot detection probe and the capture probe to form a sandwich structure, and the immune complex is enriched by a magnetic separator, The quantum dot fluorescent signal on the surface of the magnetic beads can quantify the target protein.The detection sensitivity of the method is 38 pg / mL and the linear range is 0.39 ~ 50ng / mL. The test results of clinical quality control samples show that the method has high accuracy , Which has good reproducibility and can be used in clinical samples detection.The quantum dot probe detection system has the advantages of high sensitivity, good specificity and low sample consumption, and has broad application prospects in the early diagnosis of the disease.