Ozone is one of the key species in the processes of atmospheric chemistry, which can be taken as an indicator of oxidation capacity in the troposphere, The reaction of ozone with reactive gases is an important process in the troposphere. Experimental simulation equipment of smog chamber for atmospheric reactions is used to study the reaction of ozone with ethylene in real atmospheric environment with ozone concentrations of 100-200 ppb. The concentrations of ozone and ethylene were monitored during the reaction with the combination of Model 49C-O3 Analyzer and GC-FID. A rate constant of 1.01×10-18 (cm3.mol-1.s-1) was obtained at 286.5 K,under condition of which the half-life of ozone was 88 min. The results obtained from our experiments are in excellent agreement with those reported previously by other researchers under extremely Iow pressure in terms of matrix-isolation technology. This demonstrates that our equipment of smog chamber for atmospheric reactions is reliable, which can be used for further research of the processes of atmospheric reactions.