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观察白细胞介素 - 16 (interleukin- 16 ,IL- 16 )在脓毒症时肝和肺组织中的细胞定位及分布 ,并探讨其在脓毒症发病中的作用和意义。应用新型敏感的免疫组织化学方法 (超敏感 - SP法 )检测本校近年收集的 18例感染、败血症和脓毒症尸检肝和肺组织白细胞介素 - 16蛋白的原位表达。在肝组织中 IL- 16主要定位于肝窦枯否细胞、单核细胞以及部分中性粒细胞 ,阳性率达 72 .3%。肝组织形态改变主要包括肝窦扩张充血 ,枯否细胞增生肿胀 ,肝细胞变性及不同程度的灶性坏死。在肺组织中 IL- 16定位于肺间质及感染灶内的巨噬细胞 ,中性粒细胞以及少量淋巴细胞 ,其阳性率达 6 6 .7%。肺组织形态改变主要包括肺泡壁毛细血管扩张充血 ,间质及肺泡壁内大量炎性细胞浸润 ,部分肺泡扩张 ,肺泡壁断裂 ;可见灶性坏死 ,坏死区肺结构消失 ,纤维组织增生。同时 ,观察到 IL- 16随组织病理变化的加重表达增强的趋势。本研究结果提示 IL- 16可能在脓毒症所致脏器损害中发挥重要作用。
To observe the cell location and distribution of interleukin-16 (IL-16) in liver and lung tissues during sepsis and to explore its role and significance in the pathogenesis of sepsis. The expression of interleukin - 16 (IL - 16) in liver and lung tissues of 18 infected, septicemia and sepsis autopsy specimens collected in recent years was detected by a novel and sensitive immunohistochemical method (hypersensitivity - SP method). In the liver tissue IL-16 mainly located in the liver sinus Kupffer cells, monocytes and some neutrophils, the positive rate of 72.3%. Liver morphology changes include sinusoidal dilatation and congestion, Kupffer cell hyperplasia, hepatocellular degeneration and varying degrees of focal necrosis. In the lung tissue IL-16 located in the lung interstitium and infection of macrophages, neutrophils and a small amount of lymphocytes, the positive rate of 6.67%. The morphological changes of lung tissue mainly include the expansion and congestion of alveolar capillaries, a large number of infiltration of inflammatory cells in the interstitium and alveolar wall, the expansion of part of alveoli and the rupture of alveolar wall. Visible focal necrosis, disappearance of lung structure in necrotic area and proliferation of fibrous tissue. At the same time, IL-16 was observed to increase with the pathological changes of the expression of the trend. Our results suggest that IL-16 may play an important role in the pathogenesis of sepsis-induced organ damage.