论文部分内容阅读
目的检测RhoA和Snail在胃癌组织中的表达,以探讨它们与胃癌生物学行为的关系。方法采用免疫组织化学方法检测189例胃癌患者术后石蜡标本中RhoA和Snail蛋白的表达,分析它们的相关性以及与胃癌临床病理学特征和生存的关系。结果①RhoA和Snail在胃癌组织中的表达阳性率均分别明显高于其在癌旁组织(RhoA:P=0.008;Snail:P=0.000)和正常胃黏膜组织(RhoA:P=0.010;Snail:P=0.000)中的表达阳性率;RhoA在癌旁组织中的表达与其在正常胃黏膜组织中的表达差异无统计学意义(P=0.782),而Snail在癌旁组织中的表达明显高于其在正常胃黏膜组织中的表达,差异有统计学意义(P=0.001)。②RhoA在胃癌组织中的阳性表达与胃癌的TNM分期和Lauren分型有关(P<0.05),而与胃癌的肿瘤直径、有无淋巴结转移及分化程度无关(P>0.05)。Snail在胃癌组织中的阳性表达与胃癌肿瘤直径、有无淋巴结转移、分化程度、TNM分期及Lauren分型均有关(P<0.05)。RhoA和Snail在胃癌组织中的阳性表达均与患者的性别和年龄无关(P>0.05)。③胃癌组织中RhoA蛋白和Snail蛋白的表达呈显著正相关(rs=0.203,P=0.005)。④肿瘤TNM分期、RhoA及Snail表达和淋巴结转移均为胃癌术后患者的独立预后因素(P<0.05)。结论 RhoA和Snail在胃癌组织中表达上调,它们可能共同参与了胃癌的发生、发展过程,且RhoA/Snail信号途径可能在胃癌的浸润和转移过程中发挥着重要作用。
Objective To detect the expression of RhoA and Snail in gastric cancer tissues and to explore their relationship with the biological behavior of gastric cancer. Methods The expressions of RhoA and Snail proteins in paraffin-embedded specimens of 189 patients with gastric cancer were detected by immunohistochemistry. Their correlations were analyzed. The correlations were also analyzed with the clinicopathological features and survival of gastric cancer. Results (1) The positive rates of RhoA and Snail in gastric cancer tissues were significantly higher than those in adjacent tissues (RhoA: P = 0.008; Snail: P = 0.000) and normal gastric mucosa (RhoA: = 0.000). The expression of RhoA in adjacent non-cancerous tissues was not significantly different from that in normal gastric mucosa (P = 0.782), while the expression of Snail in paracancerous tissues was significantly higher than that In normal gastric mucosa, the difference was statistically significant (P = 0.001). (2) The positive expression of RhoA in gastric cancer was related to the TNM staging and Lauren classification of gastric cancer (P <0.05), but not to the tumor diameter, lymph node metastasis and differentiation (P> 0.05). The positive expression of Snail in gastric cancer was related to tumor diameter, lymph node metastasis, differentiation, TNM stage and Lauren classification (P <0.05). The positive expression of RhoA and Snail in gastric cancer tissues was not related to the gender and age of the patients (P> 0.05). ③ There was a significant positive correlation between RhoA protein and Snail protein in gastric cancer (rs = 0.203, P = 0.005). ④ TNM stage, RhoA and expression of Snail and independent prognostic factors (P <0.05) were postoperative gastric cancer lymph node metastasis. Conclusions RhoA and Snail are upregulated in gastric cancer. They may participate in the carcinogenesis and progression of gastric cancer. RhoA / Snail signaling pathway may play an important role in the invasion and metastasis of gastric cancer.