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为了探讨烟粉虱Bemisia tabaci不同种群个体乙酰胆碱酯酶敏感性差异及其与抗药性的关系,我们选用室内饲养的烟粉虱SUD-S敏感品系和6个田间抗性种群,采用酶标板酶动力学法测定了各品系(种群)乙酰胆碱酯酶对抑制剂的敏感性反应以及抑制剂存在时各抗性种群个体乙酰胆碱酯酶残余活性频率分布。结果表明:在抑制剂浓度为300μmol/L时,敏感品系乙酰胆碱酯酶的活性基本上被完全抑制,可以明显地区分敏感品系与田间抗性种群。在抑制剂浓度为2000μmol/L时,各抗性种群个体乙酰胆碱酯酶残余活性频率分布差异明显,其中ZZ-R种群和FZ-R种群的乙酰胆碱酯酶残余活性频率分布相似,大部分个体的乙酰胆碱酯酶残余活性分布在1.00～1.80mOD/min之间;SM-R种群和ND-R种群的乙酰胆碱酯酶残余活性频率分布也相似,大部分个体的乙酰胆碱酯酶残余活性分布在0.40～1.00mOD/min之间;LY-R和NP-R种群大部分个体的乙酰胆碱酯酶残余活性分别分布在1.00～1.60mOD/min和0.80～1.20mOD/min之间。各抗性种群乙酰胆碱酯酶高残余活性(大于1.00mOD/min)个体频率与对敌敌畏的抗性水平之间具有明显相关性,相关系数为0.86(P<0.05)。考虑到乙酰胆碱酯酶对抑制剂作用不敏感是一些昆虫对有机磷和氨基甲酸酯类杀虫剂抗性的重要机制之一,建议可以将乙酰胆碱酯酶对敌敌畏的敏感性作为烟粉虱抗药性生化检测的一个参考指标。
In order to investigate the difference of acetylcholinesterase (AchE) susceptibility among different populations of Bemisia tabaci and its relationship with drug resistance, we selected SUD-S sensitive strains and 6 field resistant populations of B. tabaci, Kinetic method was used to determine the sensitivity of each strain (population) of acetylcholinesterase to inhibitor and the frequency distribution of acetylcholinesterase residual activity in each resistant population in the presence of inhibitor. The results showed that at the inhibitor concentration of 300 μmol / L, the activity of acetylcholinesterase in the susceptible strain was completely inhibited, and the susceptible strain and the field resistant population could be clearly distinguished. At the inhibitor concentration of 2000μmol / L, there was significant difference in the residual activity frequency distribution of acetylcholinesterase in each resistant population. Among them, the frequency distribution of acetylcholinesterase residual activity was similar in ZZ-R and FZ-R populations. Most of the individuals had acetylcholine The residual activities of esterase ranged from 1.00 to 1.80mOD / min. The frequencies of acetylcholinesterase residual activity in SM-R and ND-R populations were also similar. The residual activities of acetylcholinesterase in most individuals ranged from 0.40 to 1.00 mOD / min. The residues of acetylcholinesterase in most individuals of LY-R and NP-R populations were distributed between 1.00-1.60mOD / min and 0.80-1.20mOD / min, respectively. There was a significant correlation between individual frequency of high residual acetylcholinesterase (> 1.00mOD / min) and resistance to dichlorvos in each resistant population, with a correlation coefficient of 0.86 (P <0.05). Considering that insensitivity of acetylcholinesterase to inhibitors is one of the important mechanisms of insect resistance to organophosphorus and carbamate insecticides, it is suggested that the sensitivity of acetylcholinesterase to dichlorvos can be taken as the resistance to B. tabaci Biochemical detection of a reference index.