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采用免疫组织化学方法分析食管癌组织和癌旁组织中肿瘤抑制基因Ｐ１６的变化，并与肿瘤抑制基因Ｐ５３蛋白聚集的变化进行比较研究。结果：２２例食管鳞癌组织中，３例出现Ｐ１６蛋白的免疫阳性反应（１４％），此３例均为高分化鳞癌。在３例Ｐ１６免疫阳性组织中，２例同时出现Ｐ５３蛋白聚集的改变。２２例手术切除癌标本中的各级癌前病变均未出现Ｐ１６阳性反应。癌组织和癌旁上皮各级病变均出现较高的Ｐ５３蛋白改变。提示：肿瘤抑制基因Ｐ１６的变化可能主要发生在食管癌变的终末阶段，Ｐ１６与Ｐ５３有较高的一致性改变。
Immunohistochemical method was used to analyze the changes of tumor suppressor gene P16 in esophageal cancer tissues and paracancerous tissues, and compared with the changes of tumor suppressor gene P53 protein aggregation. RESULTS: Of the 22 esophageal squamous cell carcinomas, 3 showed positive immunoreactivity for P16 protein (14%). All 3 cases were well-differentiated squamous cell carcinomas. In 3 cases of P16 immunopositive tissue, 2 cases of P53 protein aggregation change. P16 positive reactions were not found in all the precancerous lesions in 22 surgically resected cancer specimens. High levels of P53 protein were found in all stages of cancerous tissue and paraneoplastic epithelium. It is suggested that the change of tumor suppressor gene P16 may occur mainly in the terminal stage of esophageal carcinogenesis and there is a higher consistency between P16 and P53.