论文部分内容阅读
目的 :观察肾衰宁 (SSN)灌肠液对体外培养人肾小球系膜细胞增殖及产生白介素 8(IL 8)的影响 ,从细胞生物学水平探讨 SSN防治慢性肾功能衰竭 (CRF)的作用机制。方法 :采用 4甲基偶氮唑盐 (MTT)比色法和双抗夹心酶联免疫吸附法 (EL ISA) ,观察 SSN大鼠血清对体外培养人肾小球系膜细胞增殖和 IL 8生成的影响。结果 :内毒素 (L PS)可明显刺激人肾小球系膜细胞增殖和产生 IL 8,SSN大鼠血清对 L PS刺激后人肾小球系膜细胞增殖和产生 IL 8有抑制作用 ,其抑制程度与药物浓度呈量效关系。结论 :SSN抑制系膜细胞增殖和自分泌 IL 8是预防肾小球硬化发生发展的重要作用机制之一。
OBJECTIVE: To observe the effect of Shenshaoning (SSN) enema on the proliferation of human glomerular mesangial cells and the production of interleukin 8 (IL 8) in vitro. To explore the role of SSN in preventing and treating chronic renal failure (CRF) from the level of cell biology. mechanism. METHODS: MTT colorimetric assay and double-antibody sandwich enzyme-linked immunosorbent assay (EL ISA) were used to observe the effects of SSN rat serum on the proliferation of human glomerular mesangial cells and IL-8 production in vitro. Impact. RESULTS: LPS could stimulate the proliferation of human glomerular mesangial cells and produce IL 8, SSN rat serum inhibited the proliferation of human glomerular mesangial cells and the production of IL 8 after LPS stimulation. The inhibition degree was in a dose-effect relationship with the drug concentration. Conclusion : SSN inhibits proliferation and autocrine of mesangial cells IL-8 is one of the important mechanisms to prevent the development of glomerulosclerosis.