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利用特大粒粳稻TD70(2011年千粒重达80g)和籼稻品种Kasalath杂交,经单粒传法获得的240个重组自交系(RIL)为作图群体,分别于2010年和2011年对粒长、粒宽、粒厚性状进行鉴定,用完备区间作图法,以均匀分布于12条染色体的141个SSR标记对粒型性状进行QTL检测。共检测到粒型性状的QTL 18个,分布于第2、3、5、7、9和12染色体上。其中,控制粒长的QTL 5个,控制粒宽的QTL 6个,控制粒厚的QTL 7个。两年间均能检测到的QTL有7个,分别为粒长QTL qGL3.1,粒宽QTL qGW2.1、qGW2.2、qGW5.1、qGW5.2,粒厚QTL qGT2.3、qGT3.1;其平均贡献率分别为56.19%、4.42%、29.41%、10.37%、7.61%、21.19%和17.06%。第2染色体RM1347-RM5699区间是粒长、粒宽、粒厚的共同标记区间。第3染色体RM6080-RM6832区间为粒长qGL3.1、粒厚qGT3.1共同标记区间。18个QTL的增效等位基因均来源于大粒亲本TD70,且增效作用显著。定位的大部分位点包含已报道的精细定位和克隆的主要粒型基因;除第2染色体的qGW2.1(qGT2.1)、qGW2.3、qGL2.2和第12染色体的qGT12等位点已有粒型性状相关报道外,定位的qGT2.2,qGW9和qGT9可能是新的QTL。
240 recombinant inbred lines (RILs) obtained from single-grain crosses between crossed japonica rice TD70 (2011 grain weight of 80g) and indica rice variety Kasalath were used as the mapping population. The grain length, Grain width and grain thickness traits. The complete interval mapping method was used to detect the QTLs of grain traits using 141 SSR markers evenly distributed on 12 chromosomes. A total of 18 QTLs for grain traits were detected on chromosomes 2, 3, 5, 7, 9 and 12. Among them, 5 were QTL controlling grain length, 6 QTL controlling grain width and 7 QTL controlling grain thickness. Seven QTLs could be detected in two years, which were grain length QTL qGL3.1, grain width QTL qGW2.1, qGW2.2, qGW5.1, qGW5.2, grain thickness QTL qGT2.3, qGT3.1 The average contribution rates were 56.19%, 4.42%, 29.41%, 10.37%, 7.61%, 21.19% and 17.06% respectively. The second chromosome RM1347-RM5699 interval is a common marker interval of grain length, grain width and grain thickness. The range of RM6080-RM6832 on chromosome 3 was the grain length qGL3.1 and the grain thickness qGT3.1 shared the marker interval. Eighteen QTL synergistic alleles were derived from the large-size parent TD70, and the synergistic effect was significant. Most of the loci contained the major granulotype genes that have been reported for fine mapping and cloning; qGW2.1 (qGT2.1), qGW2.3, qGL2.2 on chromosome 2 and qGT12 on chromosome 12 Already reported in grain traits, located qGT2.2, qGW9 and qGT9 may be new QTL.