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目的:探讨评估心肌缺血-再灌注损伤程度的两种染色方法。方法:大鼠冠状动脉左前降支结扎缺血30min,再灌注6h形成缺血-再灌注损伤动物模型。超声检测心功能指标,并分别用TTC单染法和Evans blue-TTC双染法评价心肌的损伤程度。结果:Evans blue-TTC双染法可以评价出心肌的缺血组织面积、梗死组织面积和正常组织面积,而TTC单染法只能测出组织的梗死面积和缺血面积,不能排除由于结扎位置不同而引起的误差。同时,双染法得出的梗死面积/危险面积的比值(I/R)与心功能指标的相关性,比单染法得出的梗死面积/左心室的面积比值(I/T)与心功能指标的相关性更高。结论:Evans blue-TTC双染法比TTC单染法能更客观更准确的反映出心肌组织缺血-再灌注损伤的程度。
Objective: To explore two kinds of staining methods to evaluate the extent of myocardial ischemia-reperfusion injury. Methods: The left anterior descending coronary artery of rats was ligated for 30 minutes and reperfusion for 6 hours to establish the model of ischemia-reperfusion injury. The cardiac function indexes were detected by ultrasound, and the degree of myocardial damage was evaluated by TTC single staining and Evans blue-TTC double staining respectively. Results: Evans blue-TTC double staining method can be used to evaluate the ischemic area, infarct area and normal tissue area of myocardium. However, the TTC single staining method can only detect the infarction area and the ischemic area of the tissue, Errors caused by different. At the same time, the ratio of infarction area / risk area (I / R) and cardiac function index obtained by double staining was significantly lower than that of infarction area / left ventricular area ratio (I / T) The relevance of functional indicators is higher. Conclusion: Evans blue-TTC double staining method can reflect the degree of myocardial ischemia-reperfusion injury more objectively and accurately than TTC single staining method.