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目的:观察左旋多巴神经毒性作用,探讨银杏叶提取物对左旋多巴神经毒性抑制作用。方法:采用帕金森病大鼠模型,通过细胞凋亡、行为学、Nissl 染色、免疫组织化学方法,选择左旋多巴(levodopa,L-dopa)治疗后3、5、7d 为观察时点,比较 L-dopa 治疗组和银杏叶提取物(ginkgo biloba extract,GBE)与 L-dopa联合治疗组(E-D 组)PD 大鼠的效果。结果:与 L-dopa 治疗组比较,E-D 组大鼠黑质细胞凋亡数、旋转行为减少,而尼氏细胞计数、碱性成纤维生长因子(basic fibroblast growth factor,bFGF)表达则增加(P<0.05)。结论:L-dopa 具有神经毒性作用,GBE 可减轻 L-dopa 的神经毒性作用。
Objective: To observe the neurotoxicity of levodopa and investigate the inhibitory effect of Ginkgo biloba extract on neurotoxicity of levodopa. METHODS: The rat model of Parkinson’s disease was used to observe the time points of apoptosis at 3, 5, and 7 days after treatment with levodopa (L-dopa), apoptosis, behavior, Nissl staining, and immunohistochemistry. Effects of L-dopa treatment group and Ginkgo biloba extract (GBE) and L-dopa combined treatment group (ED group) PD rats. RESULTS: Compared with L-dopa treatment group, the number of apoptotic cells and the rotational behavior of nigral cells in the ED group were decreased, while the Nissl cell count and the expression of basic fibroblast growth factor (bFGF) were increased. <0.05). Conclusion: L-dopa has a neurotoxic effect and GBE can reduce the neurotoxic effects of L-dopa.