论文部分内容阅读
目的 调查中国浙江人群中FCGR3B的基因频率及基因变异。方法 聚合酶链反应 序列特异性引物方法 (PCR SSP)检测FCGR3B基因型。DNA测序分析FCGR3基因变异。结果 来自浙江的 4 87名健康中国人中 ,FCGR3B 1与FCGR3B 2的基因频率分别是 0 5 6 4及 0 4 2 9,而FCGR3B 3的基因频率为 0 0 0 0。FCGR3B基因缺失 (FCGR3Bnull)的频率为 0 6 2 % (3/487)。 19名中国人中的 7人存在FCGR3基因变异 ,表现为在第 3个外显子的多态性位点 14 1、14 7、2 2 7、2 6 6和2 77中发生 1个或多个单核苷酸改变。结论 在中国浙江人群中 ,FCGR3B 1基因频率高于FCGR3B 2 ,而FCGR3B 3可能缺失。单核苷酸改变所形成的新的基因变异在中国人群中也存在 ,但其原因仍不清楚
Objective To investigate the gene frequency and genetic variation of FCGR3B in Chinese Zhejiang population. Methods Polymerase chain reaction sequence specific primers (PCR SSP) method was used to detect the genotypes of FCGR3B. DNA sequencing analysis of FCGR3 gene variation. RESULTS: Among 4 87 healthy Chinese from Zhejiang, the frequencies of FCGR3B 1 and FCGR3B 2 genes were 0 564 and 0 429, respectively. The frequency of FCGR3B 3 gene was 0 0 0 0. The frequency of FCGR3B deletion was 0 6 2% (3/487). Seven of the 19 Chinese individuals had FCGR3 gene variation characterized by one or more of the polymorphisms at the third exon 14 1,14 7,2 2 7,2 6 6 and 2 77 Single nucleotide changes. Conclusion In Zhejiang province of China, the frequency of FCGR3B 1 gene is higher than that of FCGR3B 2, but FCGR3B 3 may be deleted. The new genetic variation caused by the single nucleotide change also exists in Chinese population, but the reason is still unclear