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目的探讨FGFR4基因rs351855单核苷酸多态性与中国汉族人群前列腺癌术后生化复发的相关性。方法提取346例前列腺癌患者外周血DNA,采用Sequenom MassARRAY法检测rs351855位点基因型;收集患者临床信息并随访,分析比较生化复发组与未生化复发组之间数据;采用Kaplan-Meier生存曲线和COX回归分析基因型对术后无生化复发生存的影响。结果 346例患者中有124例(35.8%)发生术后生化复发。两组间术前前列腺特异抗原(PSA)水平、Gleason评分、TNM分期、切缘情况及rs351855基因型分布具有显著差异。生存分析显示不同基因型之间无生化复发生存差异具有统计学意义,携带AA/AG基因型个体较GG基因型具有更差的预后;多因素分析显示rs351855基因型是独立的预测术后无生化复发生存的因子(HR=1.873,95%CI:1.209~2.901,P=0.005)。结论FGFR4基因rs351855多态性位点与中国汉族人群前列腺癌术后生化复发有联系,可以作为预测术后生化复发的分子标记物,指导临床评估和随访。
Objective To investigate the association between rs351855 SNP of FGFR4 gene and biochemical recurrence of prostate cancer in Chinese Han population. Methods Genomic DNA was extracted from 346 cases of prostate cancer patients using Sequenom MassARRAY method. The clinical data of patients were collected and followed up. The data of biochemical recurrence group and non-biochemical recurrence group were analyzed. Kaplan-Meier survival curves Effect of Genotypes on Postoperative No Biochemical Recurrence by COX Regression Analysis. Results Of the 346 patients, 124 (35.8%) had postoperative biochemical recurrence. Preoperative PSA, Gleason score, TNM stage, margins and genotype distribution of rs351855 were significantly different between the two groups. Survival analysis showed that there was a statistically significant difference in survival-free survival among different genotypes, and individuals with AA / AG genotype had poorer prognosis than GG genotype. Multivariate analysis showed that rs351855 genotype was independently predictive of postoperative non-biochemical Recurrence factors (HR = 1.873, 95% CI: 1.209-2.901, P = 0.005). Conclusion The rs351855 polymorphism site of FGFR4 gene is associated with postoperative biochemical recurrence of prostate cancer in Chinese Han population. It may be used as a molecular marker to predict postoperative biochemical recurrence and guide clinical evaluation and follow-up.