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TPS基因在海藻糖的积累过程中具有决定性作用,目前对TPS亚家族Ⅰ型基因研究比较多,而对TPS亚家族Ⅱ型基因研究较少。我们选取了西瓜Ⅱ型TPS亚家族基因中的一个TPS基因,命名为Cl TPS1,对该基因进行了生物信息学分析。并进一步以二倍体西瓜细胞为材料,利用q RT-PCR技术对Cl TPS1基因在茉莉酸甲酯诱导下的表达特性进行了研究。结果表明Cl TPS1基因的转录起始位点位于起始密码子上游1 332 bp处,启动子区域包含多个激素响应元件和逆境胁迫响应元件等;该基因编码的蛋白含有831个氨基酸,分子量为94 104.2 Da,理论等电点为5.57。该基因与拟南芥TPS7基因同源性最高。而蛋白结构分析进一步暗示了该基因可能兼备TPS活性和TPP活性。在茉莉酸甲酯施加了4 h后Cl TPS1基因的表达量达到最高,整体变化呈现出先上升后下降的趋势。以上结果表明Cl TPS1基因能迅速响应逆境胁迫,并通过促进海藻糖生物合成抵御逆境。本研究不仅为利用茉莉酸甲酯提高西瓜抗逆性提供了理论支持,而且为以后深入研究植物中Ⅱ型TPS亚家族基因奠定基础。
The TPS gene plays a decisive role in the trehalose accumulation. At present, there are many studies on TPS subfamily type I genes, but few studies on TPS subfamily type II genes. We selected a TPS gene in the TPS subfamily of watermelon type Ⅱ, named Cl TPS1, and performed bioinformatics analysis of the gene. Furthermore, the expression characteristics of Cl TPS1 gene induced by methyl jasmonate were investigated by q RT-PCR using diploid watermelon cells as materials. The results showed that the transcription initiation site of Cl TPS1 gene was located 1 332 bp upstream of the start codon. The promoter region contained multiple hormone response elements and stress response elements. The protein encoded by this gene contained 831 amino acids with a molecular weight of 94 104.2 Da, the theoretical isoelectric point is 5.57. This gene has the highest homology with Arabidopsis TPS7 gene. The protein structure analysis further suggests that the gene may have TPS activity and TPP activity. The expression of Cl TPS1 gene reached the highest level after methyl jasmonate was applied for 4 h, and the overall change showed a trend of first increasing and then decreasing. The above results indicate that the Cl TPS1 gene can respond rapidly to stress and resist stress by promoting trehalose biosynthesis. This study not only provides theoretical support for the use of methyl jasmonate in improving watermelon resistance, but also lays the foundation for the further study of type II TPS subfamily genes in plants in the future.