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目的探讨大肠癌组织中annexinⅠ(AnxⅠ)的表达及意义。方法应用原位杂交和免疫组化方法,从mRNA及蛋白两个水平上检测95例大肠癌及35例正常大肠黏膜组织AnxⅠ的表达情况。结果大肠癌组AnxⅠmRNA表达较正常组下调,而大肠癌组AnxⅠ蛋白表达较正常组上调,两组差异极显著(P<0.01)。在mRNA水平,表达与肿瘤分化程度呈正相关(P<0.05),与有无淋巴结转移和组织学类型无关。在蛋白水平,表达与淋巴结转移呈正相关(P<0.05),与组织学类型和分化程度无关。原发癌与淋巴结转移癌两个水平表达差异均不显著(P>0.05)。结论AnxⅠ与大肠癌发生发展和转移密切相关,可作为反映大肠癌生物学行为和判断预后的重要指征。
Objective To investigate the expression and significance of annexin Ⅰ (AnxⅠ) in colorectal cancer. Methods The expression of AnxⅠ in 95 cases of colorectal carcinoma and 35 cases of normal colorectal mucosa was detected by in situ hybridization and immunohistochemistry from both mRNA and protein levels. Results The expression of AnxⅠmRNA in colorectal cancer group was down-regulated compared with that in normal group. However, the expression of AnxⅠ protein in colorectal cancer group was higher than that in normal group (P <0.01). At the mRNA level, the expression was positively correlated with the degree of tumor differentiation (P <0.05), but not with or without lymph node metastasis and histological type. At the protein level, there was a positive correlation between the expression and lymph node metastasis (P <0.05), but not with histological type and degree of differentiation. There was no significant difference in the two levels of primary cancer and lymph node metastasis (P> 0.05). Conclusion AnxⅠ is closely related to the development and metastasis of colorectal cancer, which may be used as an important indicator to reflect the biological behavior of colorectal cancer and prognosis.