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用免疫荧光和免疫酶标组织化学技术对大鼠实验性矽肺形成过程中的Ⅳ型胶原、层粘连蛋白(LM)和纤维连接蛋白(FN)三者的分布变化进行了动态观察。发现FN从病变初始的炎症期就出现在肺泡上皮周围炎症灶内疏散分布,随后明显增多及至包裹在所有胶原纤维的表面。LM是一种专一的基底膜成份,与FN的变化完全相反,随肺泡结构的破坏和纤维化病变的形成逐渐减少以致消失。Ⅳ型胶原也称基底膜胶原,但不是专一的基底膜成份,在肉芽肿期肺泡结构被破坏,网状纤维取而代之时,它并未减少却呈纤维走向,当病变发展成胶原纤维结节时,它才趋于消失。作者对观察到的上述现象及其在矽肺纤维化过程中的意义进行了讨论。
Immunofluorescence and immunoenzyme histochemical techniques were used to observe the changes of the distribution of collagen type Ⅳ, laminin (LN) and fibronectin (FN) during experimental silicosis in rats. It was found that the FN evacuated from the inflammatory phase of the alveolar epithelium during the initial inflammatory phase of the lesion, and then evidently increased to the extent that it was coated on the surface of all collagen fibers. LM is a specific basement membrane composition, and FN changes the exact opposite, with the destruction of the alveolar structure and the formation of fibrosis lesions gradually diminished and disappeared. Type IV collagen, also known as basement membrane collagen, but not a specific basement membrane composition in the granulomatous alveolar structure is destroyed, reticular fiber replaced by it, it did not reduce but was the fiber direction, when the lesions develop into collagen fiber nodules When it tends to disappear. The authors discuss the observed phenomena and their implications for silicotic fibrosis.