论文部分内容阅读
目的研究血管内皮生长因子(VEGF)在溃疡性结肠炎患者组织中的表达情况,探讨其在溃疡性结肠炎的发病机制和疾病发展中的作用及意义。方法选取溃疡性结肠炎患者30例,其中轻度8例,中度10例,重度12例;肠易激综合征组20例。采用免疫组化法检测各组中VEGF的表达情况,判断不同组间其表达水平是否存在差异。结果溃疡性结肠炎患者VEGF阳性细胞数为64.50±6.15,高于正常对照组VEGF阳性细胞数的27.05±4.10(P<0.01)。轻度、中度、重度的活动期溃疡性结肠炎病人的组织中,VEGF的阳性细胞数三者之间无显著差异性(P>0.05)。结论VEGF在溃疡性结肠炎患者的黏膜表达显著升高。VEGF在轻度、中度、重度的溃疡性结肠炎患者的黏膜表达无显著差异性。
Objective To study the expression of vascular endothelial growth factor (VEGF) in the tissue of patients with ulcerative colitis and to explore its role and significance in the pathogenesis and development of ulcerative colitis. Methods Thirty patients with ulcerative colitis were selected. Among them, 8 were mild, 10 were moderate, 12 were severe, and 20 were irritable bowel syndrome. Immunohistochemical method was used to detect the expression of VEGF in each group to determine whether there was a difference in the expression level between different groups. Results The number of VEGF-positive cells in patients with ulcerative colitis was 64.50 ± 6.15, which was higher than that of the normal control group (27.05 ± 4.10, P <0.01). There was no significant difference in the number of VEGF-positive cells among the mild, moderate and severe active ulcerative colitis patients (P> 0.05). Conclusion The expression of VEGF in mucosa of patients with ulcerative colitis is significantly increased. There was no significant difference in mucosal expression of VEGF between mild, moderate and severe ulcerative colitis patients.