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A single channel with a 160-Gb/s optical time-division-multiplexing (OTDM) transmission over 100 km is fabricated. With the help of 500-GHz optical sampling oscilloscopes, the fiber length is adjusted to the order of 10 m, which corresponds to the accuracy of 0.4 ps for the dispersion compensation. The dispersion map is optimized for the 100-km transmission link. A completely error-free transmission with the power penalty of 3.6 dB is achieved for 2 h without using forward error correction.