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目的 探讨 β 纤维蛋白原基因启动子区 45 5A/G多态性和血浆纤维蛋白原水平的关系以及在缺血性脑血管病中的作用。方法 应用PCR RFLP (HaeⅢ )方法对 91例脑梗死患者、74例选择性无血栓患者和 98例年轻健康献血员进行 β 纤维蛋白原基因启动子区 45 5A/G多态性分析 ;血浆纤维蛋白原水平测定使用凝血酶原时间法。计量资料间比较使用t检验 ,但由于纤维蛋白原浓度呈非正态分布 ,故检验前作对数转化 ;计数资料使用卡方检验。结果 H2等位基因频率在男性脑梗死患者明显较无血栓对照组高 (2 2 .7%和 7.1% ,χ2 =5 .5 6 ,P <0 .0 2 ) ,在女性组中差异无统计学意义 ;在无血栓的人群中 (包括无血栓对照组和年轻健康献血员 ) ,H2等位基因频率随年龄增长的分布频率是 :≤ 40岁为 2 1.3% ;41～ 5 9岁为 15 .4% ;≥ 6 0岁为 10 .0 % ;男性无血栓对照和健康献血员中 ,H1H1基因型人群血浆纤维蛋白原水平 (2 87g/L± 96g/L和 2 34g/L± 5 8g/L)明显较H1H2和H2H2型 (331g/L± 44g/L和 30 7g/L± 5 5g/L ;t=2 .5 3和 9.6 7,P <0 .0 5 )低。在女性组中尚未发现同样现象。结论 男性人群血浆纤维蛋白原水平受 β 纤维蛋白原基因启动子区 45 5A/G多态性的影响 ,H2等位基因是男性缺血性脑血管病的高危因素
Objective To investigate the relationship between 45 5A / G polymorphism in the promoter region of β-fibrinogen gene and plasma fibrinogen level and its role in ischemic cerebrovascular disease. Methods Polymorphism of 45 5A / G in the promoter region of β-fibrinogen gene was analyzed by PCR RFLP (HaeⅢ) in 91 patients with cerebral infarction, 74 patients with selective thrombosis and 98 healthy young blood donors. Plasma fibrin The original level determination using prothrombin time method. Comparison of measurement data using t test, but because of fibrinogen concentration was non-normal distribution, so before the test for logarithmic conversion; count data using the chi-square test. Results The frequency of H2 allele was significantly higher in patients with cerebral infarction than in non-thrombosis control group (22.7% vs 7.1%, χ2 = 5.56, P <0. 02) Significance of frequency of H2 alleles with age in thrombosis-free subjects (including thrombosis-free controls and young healthy blood donors) was: 21.3% ≤ 40 years old and 15% for 41-59 years .4%; ≥60 years old was 10 .0%. In the male thrombosis control group and the healthy blood donors, the level of plasma fibrinogen in the H1H1 genotypes (2 87 g / L ± 96 g / L and 2 34 g / L ± 58 g / L) was significantly lower than that of H1H2 and H2H2 types (331 g / L ± 44 g / L and 30 7 g / L ± 55 g / L; t = 2.53 and 9.67, P <0.05). The same phenomenon has not been found in the female group. Conclusion The plasma level of fibrinogen in male population is affected by the 45 5A / G polymorphism in the promoter region of β-fibrinogen gene. The H2 allele is a risk factor for male ischemic cerebrovascular disease