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使用中空纤维处理羟丙甲纤维素滴眼液,离心分离羟丙甲纤维素与苯扎溴铵,建立了HPLC法测定其中的苯扎溴铵含量。采用C_(18)色谱柱,流动相为70 mmol/L乙酸铵溶液(含1%三乙胺,用冰乙酸调至pH 5.0)-乙腈(30:70),检测波长262 nm。苯扎溴铵在12.5～200μg/ml浓度范围内线性关系良好,回收率为99.1%～100.3%。
Hollow fiber was used to treat hypromellose eye drops, and hypromellose and benzalkonium bromide were separated by centrifugation. The content of benzalkonium bromide was determined by HPLC. Using a C18 column, the mobile phase was 70 mmol/L ammonium acetate (containing 1% triethylamine, adjusted to pH 5.0 with glacial acetic acid)-acetonitrile (30:70), and the detection wavelength was 262 nm. The benzalkonium bromide has good linearity in the concentration range of 12.5-200 μg/ml, and the recovery rate is 99.1%-100.3%.