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目的探讨血管紧张素转换酶（ACE）基因插入/缺失（I/D）多态性与2型糖尿病伴高血压之间的相互关系。方法以ACE基因为候选基因,应用PCR方法,检测用例2型糖尿病伴高血压患者及117例2型糖尿病无高血压患者的ACE基因。结果2组研究对象DD、DⅠ、Ⅱ基因型分布频率分别为27.16％vs 20.51％、41.98％vs 41.88％、30.86％vs37.61％,D、I等位基因携带率分别为48.15％vs41.45％、51.85％vs58.55％,均有显著性差异（P<0.05）,提示ACE基因DD型及D等位基因与2型糖尿病并发高血压的发生有相关性。糖尿病患者中ACE基因I/D多态呈DD型者发生高血压的风险率为Ⅱ型者的1.32倍,且糖尿病伴高血压患者病程显著延长。正常血压糖尿病患者ACE基因 I/D多态性与单纯收缩压（SBP）升高患者ACE基因I/D多态性之间无显著差异,而与单纯舒张压（DBP）升高患者ACE基因I/D多态性之间显著相关。结论ACE基因I/D多态性参与2型糖尿病伴高血压的发病,D等位基因可能是2型糖尿病并发高血压的一个危险因子,且DBP升高在糖尿病伴高血压的发生中起重要作用。
Objective To investigate the relationship between ACE gene insertion / deletion (I / D) polymorphism and type 2 diabetes with hypertension. Methods The ACE gene was used as candidate gene and ACE gene was detected by polymerase chain reaction (PCR) in patients with type 2 diabetes mellitus and in type 2 diabetes mellitus without hypertension. Results The frequencies of DD, DⅠ and Ⅱ genotypes in the two groups were 27.16% vs 20.51%, 41.98% vs 41.88%, 30.86% vs 37.61%, respectively. The frequencies of D and I alleles were 48.15% vs 41.45 %, 51.85% vs58.55%, respectively (all P <0.05), suggesting that the DD genotypes and D allele of ACE gene are associated with the occurrence of type 2 diabetes complicated with hypertension. Diabetic patients with ACE gene I / D polymorphism were DD type of high risk of hypertension was 1.32 times the risk of type Ⅱ, and the duration of diabetes patients with hypertension significantly prolonged. There was no significant difference in ACE gene I / D polymorphism between ACE gene I / D polymorphism and elevated systolic blood pressure (SBP) in normotensive diabetic patients, but not with ACE gene I / D polymorphism between the significant correlation. Conclusion ACE gene I / D polymorphism is involved in the pathogenesis of type 2 diabetes with hypertension. D allele may be a risk factor for type 2 diabetes complicated with hypertension, and elevated DBP plays an important role in the development of diabetes with hypertension effect.