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Objective:To study effects of the exogenous bone mesenchymalstem cells(BMSCs)transplantation on silicosis fibrosisin rats,and to explore dose-effect relationship.Methods:BMSCs were isolated and cultured from male 5-week-old SD rats in vitro.Fifty healthy female SD rats were randomly divided into five groups:model control group,silicosis model group,BMSCs treatment A group(1×106/ml),BMSCs treatment B group(3×106/ml),BMSCs treatment C group(5×106/ml)(n=10).The silicosis model were made by One-time infusion of silica dust suspension,using the non-exposed tracheal intubation,and given different doses of BMSCs as intervention therapy.All the rats were sacrificed on the 21st day after model estalishment.The morphological changes of lung tissue were observed by HE staining and Masson staining;the localization and distribution of TNF-αand TGF-βwere detected by immunohistochemistry;the expression quantity of TNF-α,TGF-β,collagen I and collagen III protein were detected by Western blotting;the sex-determining gene SRY was searched by immunofluorescence to confirme BMSCs home to damage lung tissue.Results:Compared with the model control group,the silicosis model group had significantly alveolitis changes,silicon nodule formation,collagen deposition and other pathological changes.Compared with silicosis model group,the pathological changes above in BMSCs treatment A group were improved;the condition of BMSCs treatment B group was improved significantly;the pathological changes of BMSCs treatment C group were increased obviously.the expression of TNF-α,TGF-β,collagen I and collagen III protein in lung tissue:BMSCs treatment C group>silicosis model group>BMSCs treatment A group>BMSCs treatment B group>model control group.The difference of BMSCs treatment C group and silicosis model group had no statistically significant(P>0.05),and the difference between the other groups had sta-tistically significant(P<0.05).The SRY-positive cells were observed in BMSCs treatment B group by laser scanning confocal microscope,but no significant expression in the heart,liver,spleen and kidney tissues.Conclusions:The Exogenous BMSCs transplantation antagonize rat silicosis fibrosis,which has dose-effect relationship.Our data suggested that the best transplantation dose for silicosis fibrosis is 3×106/ml.
Objective: To study effects of the exogenous bone mesenchymal stem cells (BMSCs) transplantation on silicosis fibrosis in rats, and to explore dose-effect relationship. Methods: BMSCs were isolated and cultured from male 5-week-old SD rats in vitro. Fifty healthy female SD rats were divided into five groups: model control group, silicosis model group, BMSCs treatment A group (1 × 106 / ml), BMSCs treatment B group (3 × 106 / ml) ml) (n = 10). The silicosis model were made by One-time infusion of silica dust suspension, using the non-exposed tracheal intubation, and given different doses of BMSCs as an intervention therapy. All rats were sacrificed on the 21st day after model estalishment. The morphological changes of lung tissue were observed by HE staining and Masson staining; the localization and distribution of TNF-α and TGF-βwere detected by immunohistochemistry; the expression quantity of TNF-α, TGF-β, collagen I and collagen III protein were detected by Western blottin g; the sex-determining gene SRY was searched by immunofluorescence to confirme BMSCs home to damage lung tissue. Results: Compared with the model control group, the silicosis model group had significantly alveolitis changes, silicon nodule formation, collagen deposition and other pathological changes. Compared with silicosis model group, the pathological changes above in BMSCs treatment A group were improved; the condition of BMSCs treatment B group was improved significantly; the pathological changes of BMSCs treatment C group were significantly changed. β, collagen I and collagen III protein in lung tissue: BMSCs treatment C group> silicosis model group> BMSCs treatment A group> BMSCs treatment B group> model control group. The difference of BMSCs treatment C group and silicosis model group had no different significant (P> 0.05). And the difference between the other groups had sta-tistically significant (P <0.05). The SRY-positive cells were observed in BMSCs treatment B grou p by laser scanning confocal microscope, but no significant expression in the heart, liver, spleen and kidney tissues. Conclusions: The Exogenous BMSCs transplantation antagonize rat silicosis fibrosis, which has dose-effect relationship. Our data suggested that the best transplantation dose for silicosis fibrosis is 3 × 106 / ml.