论文部分内容阅读
由于同位素标记探针特异性强 ,灵敏度高 ,一直是基因表达实验研究中常用的标记物 ,我们在检测大鼠肢体缺血再灌后不同时点 ,肺组织氧合血红素酶 (ＨＯ - 1)的表达时 ,用 [α - 3 2 Ｐ]ｄＣＴＰ标记ＨＯ - 1探针 ,并于标记后进行探针纯化。现报道其效果与不纯化探针的比较。材
Because of the high specificity and high sensitivity of isotope labeled probes, they have always been the most commonly used markers in gene expression experiments. We detected the levels of HO - 1 ), The HO - 1 probe was labeled with [α - 3 2 P] dCTP and the probe was purified after labeling. Now report its effect and impure probe comparison. material