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目的探讨IL-6对肥大细胞Toll样受体4(TLR4)表达的调节作用。方法不同浓度IL-6及其阻断抗体作用P815细胞2、6、16h后,采用流式细胞术和实时定量PCR检测TLR4表达变化,ELISA法检测细胞因子释放情况。结果 IL-6呈剂量和时间依赖性地上调P815细胞TLR4的表达及细胞因子IL-4的释放,而IL-6阻断抗体能阻断这个增加过程。结论 IL-6促进TLR4的表达及细胞因子的释放,为进一步研究肥大细胞TLR4参与变态反应提供了新思路。
Objective To investigate the regulatory effect of IL-6 on the expression of Toll-like receptor 4 (TLR4) in mast cells. Methods P815 cells were treated with different concentrations of IL-6 and its blocking antibodies for 2, 6 and 16 hours. The expression of TLR4 was detected by flow cytometry and real-time PCR. The release of cytokines was detected by ELISA. Results IL-6 up-regulated TLR4 expression and IL-4 release in P815 cells in a dose-and time-dependent manner, while IL-6 blocking antibody blocked this increase. Conclusion IL-6 can promote the expression of TLR4 and the release of cytokines, which provides a new idea for further studying the involvement of TLR4 in mast cells in allergic reactions.