目的 :观察钙调素拮抗剂三氟拉嗪 (TFP)及钙通道阻滞剂异博定 (VP)对缺钙 -复钙损伤心肌的保护作用。方法 :应用TFP与VP对离体大鼠心脏进行灌流。检测心肌钙含量、灌流液中乳酸脱氢酶 (LDH)及心肌蛋白漏出量等指标。结果 :TFP显著减少损伤心肌的钙含量、LDH及蛋白漏出量 ,与对照组相比差异显著 (P <0 0 1)。VP灌注组与对照组相比各项指标差异无显著 (P >0 0 5 )。TFP与VP联合应用各项检测指标值明显低于单独应用TFP(P <0 0 1)。结论 :TFP有效减轻钙反常心肌损伤的程度 ;VP对钙反常心肌无保护作用。联合应用TFP与VP的心肌保护效果更佳。钙反常时Ca2 +大量内流主要通过钙调素介导 ,但慢钙通道在钙超载形成过程中也可能起一定作用 Objective: To observe the protective effects of calmodulin antagonist trifluoperazine (TFP) and calcium channel blocker verapamil (VP) on myocardial injury induced by calcium-deprivation. Methods: Isolated rat heart was perfused with TFP and VP. Cardiac calcium content, lactate dehydrogenase (LDH) in perfusion fluid and myocardial protein leakage were measured. Results: TFP significantly reduced the calcium content, LDH and protein leakage in injured myocardium compared with the control group (P <0.01). Compared with the control group, VP perfusion group showed no significant difference (P> 0.05). TFP and VP combined application of the detection index value was significantly lower than TFP alone (P <0 01). Conclusion: TFP can effectively reduce the degree of myocardial damage caused by calcium abnormality; VP has no protective effect on calcium abnormal myocardium. The combination of TFP and VP myocardial protection better. Ca2 + influx is mainly mediated by calmodulin, but calcium channel may also play a role in the formation of calcium overload