论文部分内容阅读
应用细胞培养、核酸斑点杂交、~３Ｈ－胸腺嘧啶核苷（~３Ｈ－ＴｄＲ）掺入、免疫细胞化学、图像分析等技术观察ｃ－ｍｙｃ反义寡核苷酸（ＯＤＮｓ）对低氧内皮细胞条件培养液（ＨＥＣＣＭ）诱导的大鼠肺血管周细胞ｃ－ｍｙｃ ｍＲＮＡ表达、增殖细胞核抗原（ＰＣＮＡ）表达及~３Ｈ－ＴｄＲ掺入量的影响。结果表明，ＨＥＣＣＭ显著促进周细胞 ｃ－ｍｙｃ ｍＲＮＡ表达、ＰＣＮＡ表达（Ｐ＜０．０１）及~３Ｈ－ＴｄＲ掺入量增加（Ｐ＜０．０１），反义ＯＤＮｓ显著阻抑 ＨＥＣＣＭ诱导的周细胞ｃ－ｍｙｃ ｍＲＮＡ表达、ＰＣＮＡ表达（Ｐ＜０．０１）及~３Ｈ－ＴｄＲ掺入量增加（Ｐ＜０．０５），而同义ＯＤＮｓ无上述作用（Ｐ＞０．０５）。结果提示，ＨＥＣＣＭ可通过上调ｃ－ｍｙｃ基因表达促进肺血管周细胞增殖，反义ＯＤＮｓ通过下凋ｃ－ｍｙｃ基因表达，抑制ＨＥＣＣＭ诱导的肺血管周细胞增殖。
The effects of c-myc antisense oligodeoxynucleotides (ODNs) on hypoxia endothelial cells (HUVECs) were observed by cell culture, dot blot hybridization, ~ 3H-thymidine incorporation, immunocytochemistry and image analysis. Effects of HECCM on c-myc mRNA expression, PCNA expression and 3H-TdR incorporation in rat pulmonary pericytes. The results showed that HECCM significantly promoted the expression of c-myc mRNA, the expression of PCNA (P <0.01) and the increase of 3H-TdR incorporation (P <0.01) The expression of c-myc mRNA, PCNA (P <0.01) and the increase of 3H-TdR incorporation (P <0.05), while the ODNs did not. The results suggest that HECCM can promote pulmonary vascular cell proliferation by up-regulating the expression of c-myc gene. Antisense ODNs inhibit the proliferation of pulmonary vascular cells induced by HECCM by down-regulating c-myc gene expression.