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目的:建立黄连解毒凝胶的定量方法。方法:采用高效液相色谱法对栀子苷、盐酸小檗碱、黄芩苷进行含量测定;均采用luna C18(4.6 mm×250 mm,5μm)色谱柱;栀子甘流动相乙腈-水(13∶87);盐酸小檗碱流动相乙腈-0.1MK2HPO4(30∶70)水溶液;黄芩苷流动相甲醇-水-磷酸(47∶53∶0.2)。结果:黄连解毒凝胶中栀子苷平均含量不少于0.4%,平均回收率97.21%,RSD 1.5%;盐酸小檗碱平均含量不低于1.2%,平均加样回收率99.11%,RSD 2.45%;黄芩苷平均含量不低于黄芩苷含量不低于1.40%,平均加样回收率98.74%,RSD 1.60%。结论:该质量标准可较好控制黄连解毒凝胶质量。
Objective: To establish a quantitative method of berberine detoxification gel. Methods: The contents of geniposide, berberine hydrochloride and baicalin were determined by high performance liquid chromatography (HPLC). All the samples were separated on a Luna C18 column (4.6 mm × 250 mm, 5 μm) : 87); berberine hydrochloride mobile phase acetonitrile -0.1MK2HPO4 (30:70) aqueous solution; baicalin mobile phase methanol-water-phosphoric acid (47:53:0.2). Results: The average content of geniposide in Coptis chinensis detoxification gel was not less than 0.4%, the average recovery was 97.21% and the RSD was 1.5%. The average content of berberine hydrochloride was not less than 1.2%, the average recovery was 99.11%, the RSD was 2.45 %; The average content of baicalin not less than baicalin content of not less than 1.40%, the average recovery rate of 98.74%, RSD 1.60%. Conclusion: This quality standard can better control the quality of Coptis detoxification gel.