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1.小鼠宫颈未分化鳞状细胞癌(U_(14))悬液接种皮下后瘤结的生长可分为三个阶段,即潜伏期(接种后3—5天瘤结开始摸到为止),瘤结开始逐渐长大(第6—11天)和12天以后,瘤结迅速长大(12天以后)。 2.传代用0.1毫升1:10的瘤组织悬液接种于昆明小鼠腋窝皮下,成活率为96.7％,未见瘤结自行消退。“接种不长”占3.3％。 3.宿主寿命为19—37天,平均寿命为27天,中间存活时间为26天。 4.癌细胞呈弥漫分布,癌细胞为多边形,细胞界限清楚,细胞间可见间隙,难见间桥,核大,呈圆、椭圆或不整形,核仁粗大,1—3个不等。瘤细胞的异型性显著。瘤间质极少。主要内脏器官与区域淋巴结很少看到转移。 5.接种初期在肿瘤实质中,可见较多的白细胞,淋巴细胞与巨噬细胞的聚集。1例接种肿瘤后无瘤生长之皮下部位,有较多的前浆细胞及浆细胞的存在,是机体免疫反应的表现。 6.本瘤已经近20年的传代,目前已传至第106代,生长与瘤型已告十分稳定。 7.本瘤株对化疗药物环磷酰胺十分敏感,其瘤重抑制率为100％,对氟尿嘧啶具有一定的敏感性。两个不同剂量的抑制率均为66％,这说明使用本瘤株作为抗癌药物的筛选是可取的。从1969年分院迁到四川后,目前传至106代。
1. The growth of mouse cervical undifferentiated squamous cell carcinoma (U_(14)) suspension inoculated subcutaneously can be divided into three stages, namely the incubation period (3 to 5 days after inoculation). The tumours began to grow (days 6-11) and after 12 days, the knobs grew rapidly (after 12 days). 2. Passaging subcutaneously into the axillary fossa of Kunming mice with 0.1 ml of 1:10 tumor tissue suspension, the survival rate was 96.7%, and no tumor regression spontaneously. “Inoculation is not long” accounts for 3.3%. 3. The host life is 19-37 days, the average life span is 27 days, and the intermediate survival time is 26 days. 4. Diffuse distribution of cancer cells, cancer cells are polygonal, cell boundaries clear, visible gaps between cells, difficult to see between the bridge, nuclear large, round, oval or not plastic, nucleolus thick, 1-3 range. The atypicality of tumor cells is significant. Very few interstitial tumors. The major visceral organs and regional lymph nodes rarely see metastases. 5. At the initial stage of inoculation, in the tumor parenchyma, more leukocytes, lymphocytes and macrophages accumulated. One subcutaneous site after tumor-inoculated tumor-free growth, with more preplasma cells and plasma cells, is the manifestation of the body’s immune response. 6. The tumor has been passaged for nearly 20 years and has now been passed to the 106th generation. Growth and tumor types have been stable. 7. The tumor strain is very sensitive to the chemotherapy drug cyclophosphamide, its tumor weight inhibition rate is 100%, has a certain sensitivity to fluorouracil. The inhibition rate was 66% at two different doses, indicating that the use of this tumor strain as an anti-cancer drug screening is desirable. After moving from the branch in 1969 to Sichuan, it has now reached 106 generations.