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目的调查配偶之间人类血小板抗原(HPA)的不配合率并评估其在新生儿同种免疫性血小板减少症(NAIT)中的作用。方法采用SSP法对200对广州汉族配偶进行了HPA-1～-16基因分型。结果配偶双方HPA-1～-6及HPA-15均在同一HPA位点具有不同基因型,HPA-7～-14及HPA-16均为a/a纯合子。得到HPA-1～-16的等位基因频率,HPA-15、HPA-3、HPA-2、HPA-6、HPA-5、HPA-1和HPA-4的不配合率为别为37.42%、37.02%、8.02%、3.84%、2.44%、0.75%和0.24%,其余HPA位点的不配合率为0。结论 HPA-5可能是广州汉族人群NAIT最重要的一个HPA系统,HPA-15、HPA-3、HPA-2、HPA-6和HPA-4也分别具有其免疫学意义,这为HPA的抗体检测以及NAIT的诊断治疗提供了实验依据。
Objective To investigate the mismatch of human platelet antigen (HPA) between spouses and assess its role in neonatal alloimmune thrombocytopenia (NAIT). Methods SSP was used to genotype HPA-1 ~ -16 in 200 Han spouses in Guangzhou. Results Both spouses HPA-1 ~ -6 and HPA-15 had different genotypes at the same HPA loci, and both HPA-7 ~ -14 and HPA-16 were a / a homozygotes. The frequency of alleles of HPA-1 ~ -16 was obtained. The unmatched rates of HPA-15, HPA-3, HPA-2, HPA-6, HPA-5, HPA-1 and HPA-4 were 37.42% 37.02%, 8.02%, 3.84%, 2.44%, 0.75% and 0.24%, respectively. Conclusions HPA-5 may be the most important HPA system of NAIT in Guangzhou Han population. HPA-15, HPA-3, HPA-2, HPA-6 and HPA-4 also have their immunological significance respectively. As well as the diagnosis and treatment of NAIT provide an experimental basis.