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以岩黄连愈伤组织为试材,采用固体培养方法,研究不同浓度的抗坏血酸对岩黄连愈伤组织褐化及抗氧化酶活性的影响。结果表明:培养第20天添加抗坏血酸的各处理岩黄连愈伤组织的褐化率均低于对照,其中抗坏血酸浓度为175mg/L的处理岩黄连愈伤组织的褐化率最低,其褐化率仅为18.5%;在整个培养阶段,添加抗坏血酸的各处理岩黄连愈伤组织的抗氧化酶活性均比对照的高,在培养第30天各处理岩黄连愈伤组织的POD和SOD活性均为最高值,其中抗坏血酸浓度为175mg/L的处理岩黄连愈伤组织的POD和SOD活性均为最高,分别达到35.24U·g-1·min-1、162.79U·g-1;在培养第20天添加抗坏血酸的各处理CAT活性均为最大值,分别比对照高了2.28、3.21、5.33、3.51U·g-1·min-1。
The experiment was carried out by using solid culture method to study the effects of different concentrations of ascorbic acid on the browning and antioxidant enzyme activities of the callus. The results showed that the browning rates of the treatments were the same as those of the control, and the browning rate was the lowest in the treatment with 175mg / L ascorbic acid. The browning rate Only 18.5%. During the whole culture period, the activities of antioxidant enzymes in the crude callus were increased as compared with the control. The POD and SOD activities of the callus were the same The highest POD and SOD activities were the highest in the treatment of 175mg / L ascorbic acid, which reached 35.24U · g-1 · min-1 and 162.79U · g-1, respectively. Ascorbic acid treatment each day CAT activity were maximum, respectively, higher than the control 2.28,3.21,5.33,3.51U · g-1 · min-1.