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目的利用毛细管气相色谱法对中药橡胶膏剂东乐膏(大黄、乳香、没药、冰片等)进行了分析,建立东乐膏GC指纹图谱。方法样品用无水乙醇超声波提取,采用DBWAX弹性石英毛细管柱(30mm×0.25mm,0.25μm,J&WScientific),FID检测器。结果确定8个色谱峰作为特征指纹峰,并对东乐膏中的主要挥发性成分冰片的含量进行了快速分离测定,分析周期为6min,线性范围为40～400mg·L-1,线性相关系数为0.9994。结论该方法可靠、简便,可作为东乐膏的产品质量控制标准。
Objective To analyze the traditional Chinese medicine rubber ointment (rhubarb, frankincense, myrrh, borneol, etc.) by capillary gas chromatography and establish GC fingerprint of Dongle ointment. Methods Samples were extracted with absolute ethanol using a DBWAX elastic quartz capillary column (30 mm x 0.25 mm, 0.25 μm, J & WScientific), FID detector. Results Eight peaks were identified as characteristic fingerprint peaks, and the content of borneol, the main volatile component in Dongle Ointment, was determined rapidly. The analysis period was 6 min, and the linear range was 40-400 mg·L-1. Linear correlation coefficient It is 0.9994. Conclusion This method is reliable, simple and can be used as a product quality control standard for Dongle Ointment.