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隐孢子虫病是一种人畜共患性寄生虫病,临床主要表现为急性或长期的慢性腹泻,对免疫功能低下者,特别是艾滋病患者,可能是致命的.近年来的调查显示,隐孢子虫感染者愈来愈多,已经成为导致腹泻的重要原因之一.现将1997～1998年收检的慢性腹泻病例(腹泻半年以上)隐孢子虫感染情况报告如下:1 材料与方法采集就诊的慢性腹泻病例新鲜粪便和感染者静脉血标本.取少量粪便标本涂在洁净玻片上,自然干燥后,甲醇固定5min,酸性复红染色3～10min,水洗;再用(?)(H_2SO_4)=10%脱色10min,直至无色,然后水洗孔雀绿染色1min,水洗,滤纸吸干,油镜下镜检,查到隐孢子虫卵为隐孢子虫感染阳性.血清标本采用单向琼脂扩散法测定IgG、lgM、lgA和补体C_3、C_4含量.免疫扩散板由重庆医学检验试剂研究所提供.
Cryptosporidiosis is a zoonotic parasitic disease, clinical manifestations of acute or chronic chronic diarrhea, immunocompromised persons, especially AIDS patients may be fatal .In recent years, the survey showed that cryptosporidiosis More and more worms infected, has become an important cause of diarrhea.Currently collected from 1997 to 1998 cases of chronic diarrhea (diarrhea more than six months) of cryptosporidiosis are reported as follows: 1 Materials and Methods Collection of treatment Chronic diarrhea cases of fresh faeces and infected venous blood samples. Take a small amount of stool specimens coated on clean glass slides, natural drying, methanol fixed 5min, acid complex red staining 3 ~ 10min, washed; (?) (H2SO4) = 10 % Decolorization 10min, until colorless, and then washed with malachite green 1min, washed with water, filter paper dry, microscopic examination under the oil microscopy, found that Cryptosporidium parvum positive for Cryptosporidium infection Serum samples were determined by one-way agar diffusion method IgG , LgM, lgA and complement C_3, C_4 content of the immune diffusion plate provided by the Chongqing Institute of Medical Laboratory Reagents.