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目的观察槲寄生黄酮苷对心肌缺血大鼠的保护作用,并进一步明确其对血小板激活因子(PAF)的调控作用。方法结扎大鼠冠状动脉左前降支造成急性心肌梗死模型,通过观察不同剂量槲寄生黄酮苷(15、75mg/kg)及PAF受体特异性阻断剂BN52021(10mg/kg)对梗死范围的影响,对比评价槲寄生黄酮苷对缺血心肌的保护作用;分离正常大鼠心肌细胞,Fluo-3/AM荧光染色,采用共聚焦显微镜技术研究,预先给予不同剂量槲寄生黄酮苷30min后对PAF(1×10-11mol/L)诱导心肌细胞钙超载的拮抗作用。结果与模型组比较,槲寄生黄酮苷和BN52021均可缩小大鼠梗死心肌范围,降低心肌梗死大鼠血清乳酸脱氢酶(LDH),丙二醛(MDA)水平,提高血清超氧化物歧化酶(SOD)水平,并成剂量效应关系。PAF可直接诱导心肌细胞钙超载,而槲寄生黄酮苷和BN52021可直接对抗这种钙超载作用。结论槲寄生黄酮苷对心肌缺血具有保护作用,作用机制与其抑制PAF诱导心肌细胞内钙超载有关,PAF信号通路阻断药物可能成为抗心肌缺血药物的研究靶点。
Objective To observe the protective effect of mistletoe flavonol glycosides on myocardial ischemia in rats, and to further clarify its regulation of platelet activating factor (PAF). Methods The model of acute myocardial infarction was induced by ligation of the left anterior descending coronary artery in rats. The effects of mistletoe flavonoid glycosides (15,75 mg / kg) and BNF-specific antagonist BN52021 (10 mg / kg) , And compared the protective effects of mistletoe flavonoid glycosides on ischemic myocardium. The normal rat myocardial cells were isolated and stained with Fluo-3 / AM by confocal microscopy. Pretreatment with different dosages of mistletoe flavonoid glycosides for 30 min, 1 × 10-11mol / L) induced cardiomyocyte calcium overload. Results Compared with the model group, both mistletoe flavonoid glycosides and BN52021 could reduce the myocardial infarct size, decrease the levels of serum LDH, MDA and the levels of serum superoxide dismutase (SOD) levels, and dose-response relationship. PAF can directly induce calcium overload in myocardial cells, and mistletoe flavonoid glycosides and BN52021 can directly counteract this calcium overload. Conclusions The results suggest that mistletoe flavonoid glycosides have a protective effect on myocardial ischemia and its mechanism may be related to the inhibition of intracellular calcium overload induced by PAF. PAF signaling pathway blocking drugs may be the target of anti-ischemic drugs.