【摘 要】
：
From a random insertion mutant library of Synechocystis sp. PCC 6803, a mutant defective in photoautotrophic growth was obtained. The interrupted gene was ident
【机 构】
：
The State Key Laboratory of Freshwater Ecology and Biotechnology
论文部分内容阅读
From a random insertion mutant library of Synechocystis sp. PCC 6803, a mutant defective in photoautotrophic growth was obtained. The interrupted gene was identified to be sir2094 (fbpl), which encodes the fructose-l,6-biphosphatase (FBPase)/sedoheptu-lose-1,7-biphosphatase (SBPase) bifunctional enzyme (F-I). Two other independently constructed slr2094 mutants showed an identical phenotype. The FBPase activity was found to be virtually lacking in an sir2094 mutant, which was sensitive to light under mixotrophic growth conditions. These results indicate that sir2094 is the only active FBPase-encoding gene in this cyanobacterium. Inactivation of photosystem 11 by interrupting psbB in sir2094 mutant alleviated the sensitiveness to light. This report provides the direct genetic evi-dence for the essential role of F-I in the photosynthesis of Synechocystis sp. PCC 6803.
其他文献
Thick cylindrical shells under transverse loading exhibit an elephant foot buckling mode, whereas moderately thick cylindrical shells show a diamond buckling mo
Efficient numerical schemes were presented for the steady state solutions of towed marine cables. For most of towed systems, the steady state problem can be res
Based on the concept of hydraulic dissipation of kinetic energy, a novel shock wave regulator, which is composed of a damper and an externally triggered valve,
Since the empirical mode decomposition (EMD) lacks strict orthogonality, the method of orthogonal empirical mode decomposition (OEMD) is innovationally proposed
In industrial CT, it is often required to inspect large objects using short line-detectors. To acquire the complete CT data for the scanning slice of large obje
Amplified spontaneous emission (ASE) in diode laser pumped double-clad fiber power amplifiers is studied experimentally. The dependences of ASE on fiber length
Pólya分布在气候统计中常用来拟合雾、雷暴等.本文给出了Pólya分布总体在全样本场合下参数的矩估计和极大似然估计.并研究了估计的存在性,并通过大量的Monte-Carlo模拟说明
Metal transfer is an important phenomenon in metal inert gas (MIG) arc welding with longitudinal alternating magnetic field. It is of great significance to obse
A new thermodynamic expression for Gibbs free energy difference 4G between the under-cooled Iiquid and the corresponding crystals of bulk metallic glasses was d
To make the geometrical basis for soft matters with curved surfaces such as biomembranes as simple as possible, a symmetrical analytical system was developed in