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我院在临床工作中,曾用下列方法进行快速白血球分类检查,效果很好,兹介绍如下,以供参考。染色液的配制 1.准备材料:蒸发皿,磷酸钾K_2HPO_4),磷酸钠(Na_2HPO_4),伊红,美蓝。 2,配制: A液:美篮1.3克,磷酸钠5克,溶於50毫升蒸馏水中,於蒸发皿上蒸发至干燥时,加磷酸钾6.25克,然后加水500毫升,加温至色素全部溶解为止,放置至次日滤过备用。 B液:将磷酸钠5克,磷酸钾6.25克,溶於60℃加温之蒸馏水中,待其溶解後再加入伊红1.3克,完全溶解后,於次日滤过备用。 C液:4％的石炭酸水溶液。
In our clinical work, the following methods have been used for rapid white blood cell classification tests, and the results are very good. They are described below for reference. Preparation of Staining Solution 1. Preparation materials: evaporating dish, potassium phosphate K 2 HPO 4), sodium phosphate (Na 2 HPO 4 ), eosin, and methylene blue. 2, preparation: A liquid: US basket 1.3 grams, 5 grams of sodium phosphate, dissolved in 50 milliliters of distilled water, evaporated to dryness on an evaporating dish, add 6.25 grams of potassium phosphate, and then add 500 milliliters of water, warmed to all the pigment dissolved Until the next day, filter it until it is ready. Solution B: 5 g of sodium phosphate and 6.25 g of potassium phosphate were dissolved in distilled water heated at 60°C. After the solution was dissolved, 1.3 g of eosin was added. After the solution was completely dissolved, the solution was filtered overnight. Solution C: 4% aqueous carbolic acid solution.