论文部分内容阅读
目的 :查明引起此次霍乱流行的病原菌并掌握其流行特征 ,临床和病原菌生物学特点 .方法 :用现患调查方法描述此次O13 9群霍乱弧菌引起流行的发病情况 ,分析发病原因 ,应用微生物学 ,分子生物学技术对O13 9菌株进行分离、培养和测定有关毒力CT基因序列并与国际标准菌株比较 .结果 :在萍乡市安源区某酒店 9月 5-10日聚餐的 3 0 7人中检出O13 9霍乱弧菌阳性者 43人 ,确诊病人 14人 ,带菌者 2 9人 ,全部为聚餐人员 ,未聚餐者不发病 ,无家庭聚集性 ,未发生二代病人 .对此次O13 9群霍乱流行菌株毒力CT基因序列测定比较后发现与 853 (O13 9群 )、N16961(Eltor)同源性 10 0 % ,与 569B(CVC)同源性 99 7% .结论 :O13 9群霍乱弧菌污染酒店内冰柜和其内保存的熟肉制品 ,再感染聚餐人员是引起此次霍乱流行的根本原因 .此流行菌株与O1群 (EVC)遗传关系最近 ,提示需加强监测了解它是否成为当地优势菌株 ,防止它引起更大范围的流行
Objective: To identify the pathogenic bacteria causing the cholera epidemic and to master its epidemiological characteristics, biological characteristics of clinical and pathogenic bacteria.Methods: To describe the prevalence of Vibrio cholerae O13 9 epidemic by the method of prevalence survey, analyze the causes, The strains of O13 9 were isolated and cultured by microbiology and molecular biology techniques, and the CT virulence genes were sequenced and compared with those of international standard strains.Results: In the Anyang District, Pingxiang City, September 5 -10 dinner 3 0 7 out of 7 detected V13 9 Vibrio cholerae positive in 43 patients diagnosed in 14 people, carriers 29 people, all for meal staff, non-meal were not disease, no family aggregation, did not occur in the second generation of patients. The sequence comparison of the CT virulence genes of the O139 cholera epidemic strains showed that they were 100% homologous to 853 (O139), N16961 (Eltor) and 99.7% homologous to 569B (CVC) .Conclusion: O13 9 Vibrio cholerae polluted hotel freezers and cooked meats preserved therein and re-infected with food service staff was the root cause of the cholera epidemic.The genetic relationship between this epidemic strain and the O1 group (EVC) recently suggested the need to strengthen surveillance Know if it becomes The dominant strains, preventing it from causing a wider range of popular