按Ａｈｏ等的方法，给大鼠胆胰管内逆行注射牛磺胆酸钠，制成急性坏死性胰腺炎动物模型。采用ＨＥ染色及ＤＮＡ末端标记法（ＴＵＮＥＬ）观察了术后（注药后）不同时程胰腺外分泌细胞的病理组织学改变。发现牛磺胆酸钠可引起胰腺组织的坏死及凋亡，前者出现较早并随术后时间延长而逐渐加重，且伴有明显的炎症反应；后者出现略迟，占较小部分。这表明，这种胰腺炎动物模型可引起胰腺外分泌细胞的坏死及凋亡，与胰腺炎的病变程度密切相关 According to the method of Aho et al., Rats were injected with sodium taurocholate retrogradely into pancreatic and pancreatic ducts to make an animal model of acute necrotizing pancreatitis. The histopathological changes of pancreatic exocrine cells at different time points after injection were observed by HE staining and DNA end labeling (TUNEL). Found that sodium taurocholate can cause necrosis and apoptosis of pancreatic tissue, the former appeared earlier and with the extension of time after surgery and gradually increased, accompanied by a clear inflammatory response; the latter appeared slightly later, accounting for smaller parts. This shows that this animal model of pancreatitis can cause pancreatic exocrine necrosis and apoptosis, and pancreatic lesions are closely related to the degree of