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[目的]提取白花蛇舌草总黄酮,探讨白花蛇舌草总黄酮对BGC-823胃癌细胞周期和凋亡的影响。[方法]用乙醇回流法提取白花蛇舌草总黄酮,标准曲线法测定其含量,AB-8大孔吸附树脂进行纯化,然后作用于BGC-823肿瘤细胞,流式细胞仪检测FHD对细胞周期及细胞凋亡的影响。[结果]乙醇回流法提取白花蛇舌草总黄酮,含量为67.2mg/g(总黄酮/干浸膏),纯度为6.72%。AB-8大孔吸附树脂纯化白花蛇舌草总黄酮后所得精制品中总黄酮纯度约为23.82%,纯化后的总黄酮纯度提高约4倍。白花蛇舌草总黄酮组较对照组G1期细胞增加,S期细胞明显减少;实验组凋亡细胞比例明显高于对照组。[结论]AB-8大孔吸附树脂对白花蛇舌草中总黄酮具有很好的纯化富集作用;白花蛇舌草总黄酮对胃癌细胞BGC-823株的生长有明显的抑制作用,作用机制可能与抑制DNA的合成,诱导细胞凋亡有关。
[Objective] The research aimed to extract the total flavonoids of Hedyotis diffusa Willd. And explore the effect of Hedyotis diffusa Willd. On the cell cycle and apoptosis of BGC-823 gastric cancer cells. [Method] Total flavonoids of Hedyotis diffusa Willd. Were extracted by ethanol reflux method. The content of HGF was determined by standard curve method. The AB-8 macroporous resin was purified and then applied to BGC-823 tumor cells. Flow cytometry was used to detect the effect of FHD on cell cycle And the impact of apoptosis. [Result] The total flavonoids of Hedyotic diffusa were extracted by ethanol reflux method, the content was 67.2 mg / g (total flavonoids / dry extract), the purity was 6.72%. Purification of Hedyotis diffusa flavonoids by AB-8 macroporous adsorption resin purified the total flavonoids purity of about 23.82%, purity of purified flavonoids increased about 4 times. Hedyotis diffusa total flavonoids group compared with the control group G1 phase cells, S phase cells decreased significantly; experimental group of apoptotic cells was significantly higher than the control group. [Conclusion] The macroporous adsorption resin AB-8 has a good purification and enrichment effect on the total flavonoids of Hedyotis diffusa Willd. Total flavonoid of Hedyotic diffusa will obviously inhibit the growth of gastric cancer cell line BGC-823 and the mechanism of action It may be related to inhibition of DNA synthesis and induction of apoptosis.