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目的:观察特发性血小板减少性紫癜(ITP)患者调节性T细胞(Treg)和叉头状转录因子FoxP3的表达情况及地塞米松治疗前后的变化。方法:用流式细胞术检测ITP患者外周血Treg、FoxP3的表达情况并与正常人群进行比较、并同时记录大剂量地塞米松治疗前后ITP患者Treg、FoxP3的表达情况,比较激素治疗对机体免疫的影响。结果:ITP患者外周血中CD4+T细胞的比例显著低于正常组(P<0.05),CD4+CD25+Treg及CD4+CD25+T在总CD4+T细胞中所占比例也低于正常对照组(P<0.05)。ITP患者未经激素治疗前外周血中FoxP3表达比例明显低于正常对照组(P<0.05),经大剂量地塞米松治疗后Foxp3表达比例升高(P<0.05),与正常人群比较差别仍有统计学意义(P<0.05)。结论:ITP患者的Foxp3表达下降,CD4+CD25+Treg细胞患者数量减少,Treg及Foxp3在ITP的发生发展中起到重要作用,大剂量地塞米松可显著提高CD4+CD25+Treg及FoxP3的表达。
Objective: To observe the expression of regulatory T cell (Treg) and forkhead transcription factor FoxP3 in patients with idiopathic thrombocytopenic purpura (ITP) and the changes of dexamethasone before and after treatment. Methods: Flow cytometry was used to detect the expression of Treg and FoxP3 in peripheral blood of ITP patients and compared with the normal population. Meanwhile, the expression of Treg and FoxP3 in ITP patients treated with high-dose dexamethasone was recorded. Impact. Results: The proportion of CD4 + T cells in peripheral blood of patients with ITP was significantly lower than that of normal controls (P <0.05), and the proportion of CD4 + CD25 + Treg and CD4 + CD25 + T in total CD4 + T cells was also lower than that of normal controls Group (P <0.05). The proportion of FoxP3 in peripheral blood of patients with ITP before hormone therapy was significantly lower than that of the normal control group (P <0.05), and the proportion of Foxp3 expression increased after high-dose dexamethasone treatment (P <0.05) There was statistical significance (P <0.05). CONCLUSION: Foxp3 expression in ITP patients is decreased and the number of CD4 + CD25 + Treg cells is decreased. Tregs and Foxp3 play an important role in the development of ITP. High-dose dexamethasone can significantly increase the expression of CD4 + CD25 + Treg and FoxP3 .