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用ABC免疫组化法和LCA,UCHL_1,L_(26),Mac_(387),k,(?),EMA,CK和CEA等9种抗体标记了81例病理学不能确诊或分类的淋巴瘤样疾病。所有组织均用10%福尔马林固定,石蜡包埋,6μm切片。标记结果:3例确诊为未分化癌,72例为非何杰金淋巴瘤(NHL),5例为淋巴组织反应性增生,1例不能确诊。在72例NHL中,B细胞淋巴瘤49例,T细胞淋巴瘤19例,B、T细胞混合性淋巴瘤1例,组织细胞肉瘤3例。结果表明,应用可以标志石蜡切片中淋巴细胞抗原的LCA,UCHL_1,L_(26),Mac_(387)等单克隆抗体对于NHL的诊断和分型是有效的。如能应用1种以上的同类血清,结合其组织形态,可以进一步提高其诊断水平。
Ninety-one antibodies labeled with ABC immunohistochemical method and LCA, UCHL_1, L_ (26), Mac_ (387), k, (?), EMA, CK and CEA were used to identify 81 pathologically indeterminate disease. All tissues were fixed with 10% formalin, embedded in paraffin and sectioned at 6 μm. Marked results: 3 were diagnosed as undifferentiated carcinoma, 72 were non-Hodgkin’s lymphoma (NHL), 5 were reactive lymphoid tissue hyperplasia and 1 was not diagnosed. In 72 cases of NHL, 49 cases of B cell lymphoma, T cell lymphoma in 19 cases, B, T cell mixed lymphoma in 1 case, 3 cases of histiocytic sarcoma. The results showed that LCA, monoclonal antibodies such as UCHL_1, L_ (26) and Mac_ (387), which could be used to label lymphocyte antigen in paraffin section, were effective for the diagnosis and typing of NHL. If you can use more than one kind of similar serum, combined with its morphology, can further improve its diagnostic level.