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目的 研究一种新发现的抗氧化蛋白质——凋亡敏感基因(SAG)在短暂性缺氧再复氧复注血清诱导的细胞坏死和凋亡中的作用。方法 用短暂性缺氧再复氧复注血清来诱导原代培养的大鼠大脑皮质星形胶质细胞损伤,用免疫细胞化学方法检测凋亡敏感基因的表达,并作图像分析;用流式细胞仪检测胶质细胞在短暂缺氧再复氧复注血清后不同时间点的凋亡率。结果 凋亡敏感基因在缺氧15 min再复氧复注血清5 h后表达最高,在复氧复注血清16 h后恢复至对照组水平;细胞凋亡率在缺氧15 min再复氧复注血清1h时达到最高,而在再复氧复注血清5 h后降至对照组水平。结论 凋亡敏感基因具有抗凋亡的作用,可减轻星形胶质细胞的缺血再灌注损伤。
AIM To study the role of a newly discovered anti-oxidative protein, the apoptosis-sensitive gene (SAG), in the cell death and apoptosis induced by transient hypoxia and reoxygenation. Methods Primary cultured rat cerebral cortical astrocytes were induced by transient hypoxia and reoxygenation, and the expression of apoptotic genes was detected by immunocytochemistry. The expression of apoptosis-sensitive genes was detected by flow cytometry Cytometry was used to detect the apoptosis rate of glial cells at different time points after transient hypoxia, reoxygenation and reperfusion. Results Apoptosis-sensitive genes were expressed 5 h after hypoxia and reoxygenation in serum, and reached the level of control at 16 h after reoxygenation and reperfusion. The rate of apoptosis increased at 15 min after reoxygenation Note 1h serum reached the highest, and in the reoxygenation serum 5h after the fall to the control group. Conclusion Apoptosis-sensitive genes play an anti-apoptotic role in reducing ischemia-reperfusion injury of astrocytes.