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目的:建立HPLC法同时测定五味护肝咀嚼片中五味子醇甲、戈米辛J、五味子醇乙、戈米辛G、五味子酯甲、五味子甲素和五味子乙素7种木脂素类成分的含量,为制定其质量标准提供依据。方法:采用高效液相色谱法,色谱柱为Kromasil 100-5C18(250mm×4.6mm,5μm);检测波长217nm;柱温为30℃;流速为1.0mL.min-1;流动相为乙腈-水,梯度洗脱。结果:被测的7种木脂素成分分离度良好;各成分质量浓度与峰面积在测定范围内均呈良好线性关系(r>0.999 5);重现性良好;准确度(RSD)(n=9)分别为99.90%(1.63%)、100.12%(1.26%)、99.42%(1.72%)、100.30%(2.21%)、99.81%(2.07%)、99.79%(1.91%)和100.57%(1.61%)。结论:本方法简便易行,结果准确可靠,适用于本制剂中五味子木脂素类成分的同时定量分析。
OBJECTIVE: To establish a HPLC method for the simultaneous determination of seven kinds of lignan components of schisandra chinensis, gomisin J, schisandrin B, gomisin G, schisandrin, schisandrin and Schisandrin B in Wuwei Hugan chewable tablets Content, to provide the basis for the development of their quality standards. Methods: The column was Kromasil 100-5C18 (250mm × 4.6mm, 5μm). The detection wavelength was 217nm. The column temperature was 30 ℃. The flow rate was 1.0mL.min-1. The mobile phase was acetonitrile-water , Gradient elution. Results: The results showed that the seven kinds of lignans were separated well; the linearity of the mass concentration and peak area of each component was linear (r> 0.999 5); the reproducibility was good; the accuracy (RSD) = 9) were 99.90% (1.63%), 100.12% (1.26%), 99.42% (1.72%), 100.30% (2.21%), 99.81% (2.07%), 99.79% (1.91%) and 100.57% 1.61%). Conclusion: The method is simple and easy to operate, the results are accurate and reliable, and is suitable for the simultaneous quantitative analysis of components of lignans in this preparation.