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目的探讨支气管哮喘患者外周血单个核细胞(PBMCs)p38MAPK表达的变化及地塞米松(DEX)对其的影响。方法分离培养哮喘患者和正常健康者的PBMCs。分别采用蛋白质印迹和ELISA方法检测PBMCs核蛋白p38MAPK的表达及细胞上清液中IL -4、IL- 5的蛋白质浓度。结果哮喘对照组PBMCs核蛋白p38MAPK表达及细胞上清液中IL -4、IL- 5的蛋白质浓度较正常对照组显著升高(P<0. 001),DEX处理组核蛋白p38MAPK表达及细胞上清液中IL- 4、IL- 5的蛋白质浓度较哮喘对照组显著降低(P<0. 01)。通过直线相关分析发现,PB MCs核蛋白p38MAPK表达与培养上清液中IL- 4、IL- 5蛋白质含量之间均呈显著正相关(r分别=0. 71、0. 64,P均<0. 01)。结论p38MAPK可能参与支气管哮喘的病理生理过程。DEX可能通过作用于p38MAPK这一靶点发挥其抗炎效应。
Objective To investigate the changes of p38MAPK expression in peripheral blood mononuclear cells (PBMCs) of patients with bronchial asthma and the effect of dexamethasone (DEX) on it. Methods PBMCs of asthmatic patients and healthy controls were isolated and cultured. Western blotting and ELISA were used to detect the expression of p38 MAPK in nuclei of PBMCs and the protein concentration of IL-4 and IL-5 in the supernatant. Results The protein expression of p38 MAPK and the level of IL-4 and IL-5 in PBMCs of asthmatic control group were significantly higher than those in normal control group (P <0.001) Serum IL-4, IL-5 protein concentrations were significantly lower than the asthma control group (P <0.01). By linear correlation analysis, it was found that there was a significant positive correlation between PBMC nuclear protein p38MAPK expression and IL-4 and IL-5 protein contents in culture supernatant (r = 0.71,0.64, P <0 01). Conclusion p38MAPK may be involved in the pathophysiological process of bronchial asthma. DEX may exert its anti-inflammatory effect by acting on the target of p38MAPK.