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目的了解新疆南部地区蜱传虫媒病毒的种类和分布情况。方法在新疆南部地区的23个采集地36个采集点,采集蜱标本5045只,分别建立蜱标本cDNA文库,用PCR方法检测标本可能携带的病毒。结果黄病毒属引物、加利福尼亚血清组病毒引物对34份cDNA文库检测结果均为阴性;内罗毕病毒属引物和新疆出血热病毒巢式引物在巴楚县蜱标本中检测到新疆出血热(XHF)病毒核酸序列,对检测到的XHF病毒L和S片段进行系统进化分析。结论对新疆南部地区5045只蜱进行四种六对引物的PCR检测,未检出黄病毒属和加利福尼亚血清组病毒核酸序列,获得新疆出血热病毒L和S片段的核酸序列,研究了其分子流行特征。
Objective To understand the species and distribution of tick-borne arboviruses in southern Xinjiang. Methods Totally 5045 ticks were collected from 36 collection sites in 23 sites in the southern part of Xinjiang. The ticks cDNA library was established, and the possible viruses carried by the samples were detected by PCR. Results The results of the detection of 34 copies of the cDNA library of both the flavivirus primer and the California serogroup virus primer were negative. The detection of the Xinjiang hemorrhagic fever virus (XHF) in the tick specimens of Bachu county resulted from the detection of the Nero virus genomic primer and the Xinjiang hemorrhagic fever virus nested primer Nucleic acid sequences were used for phylogenetic analysis of the detected L and S fragments of XHF virus. CONCLUSION: Four pairs of primers were used to detect the 5 045 ticks in southern Xinjiang. The nucleic acid sequences of flavivirus and California serogroup were not detected, and the nucleotide sequences of L and S fragments of Xinjiang hemorrhagic fever virus were obtained. feature.