论文部分内容阅读
目的 观察抗人CD3 单抗 (aCD3 McAb)对单核细胞 (PBMC)和肿瘤组织中提取的浸润淋巴细胞 (TIL)增殖的影响。方法 将外周血中分离出的PBMC和TIL进行扩增培养 ,同时测定其活性。结果 aCD3 McAb和白细胞介素 2 (IL 2 )的浓度是包被法制备CD3 单克隆抗体激活的杀伤细胞 (CD3 AK)的主要影响因素。经选择aCD3 McAb的最适蛋白浓度为 0 .1μg/ml ,IL 2为 2 0U/ml。 结论 实验发现CD3 AK细胞的抗瘤活性明显高于LAK细胞 ,PBMC和TIL的扩增倍数明显高于常规培养的LAK细胞法。
Objective To observe the effect of anti-human CD3 monoclonal antibody (aCD3 McAb) on the proliferation of infiltrating lymphocytes (TIL) extracted from monocytes (PBMC) and tumor tissues. Methods PBMCs and TILs isolated from peripheral blood were expanded and cultured, and their activity was measured. Results The concentration of aCD3 McAb and interleukin 2 (IL 2 ) was the major contributing factor to CD3 monoclonal antibody-activated killer cells (CD3 AK). The optimal protein concentration of aCD3 McAb was chosen as 0.1 μg/ml and IL 2 was 20 U/ml. Conclusion The anti-tumor activity of CD3 AK cells was significantly higher than that of LAK cells. The amplification times of PBMC and TIL were significantly higher than that of conventional cultured LAK cells.