论文部分内容阅读
目的膀胱癌患者外周血中特异性角蛋白上皮CK—20mRNA在诊断中的意义。方法以半巢式RT-PCR检测外周血有核细胞成分中CK—20mRNA。结果91例膀胱癌中检出CK—20mRNA 37例,阳性率41%,其中证实有转移的20例中17例阳性(85%),25例健康对照组均为阴性。外周血细胞CK—20mRNA的阳性率在远处转移的病例中显著升高(P<0.01)。结论检测膀胱癌患者外周血CK—20mRNA对早期发现膀胱癌细胞的血行播散有重要参考价值。
The Significance of CK-20mRNA in Peripheral Blood of Patients with Bladder Cancer in the Diagnosis. Methods Semi-nested RT-PCR was used to detect CK-20 mRNA in peripheral blood cells. Results 37 cases of CK-20 mRNA were found in 91 cases of bladder cancer, the positive rate was 41%. Of the 20 cases confirmed metastatic, 17 cases were positive (85%) and 25 cases were negative. The positive rate of CK-20 mRNA in peripheral blood cells was significantly increased in cases of distant metastasis (P <0.01). Conclusion Detection of CK-20mRNA in peripheral blood of patients with bladder cancer has important reference value for early detection of hematogenous spread of bladder cancer cells.