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目的研究大鼠下颌下腺副交感神经节分离神经元不同P2X受体的表达。方法用全细胞膜片钳的方法对大鼠下颌下腺神经节急性分离的神经元进行了药理学鉴定。结果在所有的神经元上,ATP都可以引起一个快速反应的缓慢失活的内向电流。但只在59%的神经元上,αβmeATP也可以引起一个类似的内向电流。降低pH值及同时给予Zn2+可以增强ATP及αβmeATP诱发的电流。而Ivermectin没有作用。结论在大鼠下颌下腺神经节急性分离的神经元上,主要表达同源二聚体P2X2受体和异源二聚体P2X2/3受体。
Objective To study the expression of different P2X receptors in the parasympathetic ganglion neurons of the submandibular gland of rats. Methods Whole-cell patch-clamp method was used to identify the neurons acutely isolated from rat submandibular gland. Results In all neurons, ATP can cause a rapid response to the slow inactivation of the inward current. However, αβmeATP also elicited a similar inward current only in 59% of neurons. Lowering pH and simultaneous administration of Zn2 + can enhance ATP and αβmeATP-induced currents. Ivermectin has no effect. CONCLUSIONS: The homodimeric P2X2 receptor and the heterodimeric P2X2 / 3 receptor are predominantly expressed on neurons acutely isolated from rat submandibular gland.