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为探讨雄性大鼠去睾后骨丢失的组织学机制 ,以及应用益肾健脾胶囊和钙剂后的组织学反应。选用 10月龄大鼠 6 0只 ,行去睾术制成病理模型。随机分为正常组、病理组、益肾健脾胶囊组、益肾健脾 +钙剂组、益肾 +钙剂组和钙剂组。用药 12周后处死取L1～ 2 作不脱钙骨切片 ,测定骨形态计量学各骨量参数和表面参数。各组参数均作方差分析 ,结果①病理组骨量参数小于正常组 (P <0 .0 5 ) ;表面参数显示成骨参数减少 ,破骨参数增加 ,骨转换加快 (P <0 .0 5 )。②各中药组骨量参数高于病理组 (P <0 .0 5 ) ;表面参数得到逆转。同时可见部分成骨细胞特别活跃 ,其下类骨质高出周围静止表面。③益肾健脾 +钙剂组皮质骨厚度和骨内膜矿化率高于其他中药组及钙剂组 (P <0 .0 5 )。表明重建负平衡和骨高转换是导致雄性大鼠去睾后骨丢失的组织学机制。益肾、健脾、补钙同时应用具有抑制骨吸收 ,促进骨形成 ,降低骨转换 ,提高机体对钙剂的吸收利用度 ,从而影响骨矿化过程 ,是治疗老年男性骨质疏松症的最佳方法
To investigate the histological mechanism of bone loss after decapitalization in male rats and the histological response after application of Yishen Jianpi Capsules and calcium. Sixty rats at 10 months of age were used to make pathological models. They were randomly divided into normal group, pathological group, Yishen Jianpi capsule group, Yishen Jianpi + calcium group, Yishen + calcium group and calcium group. After 12 weeks of dosing, L1～2 were sacrificed for decalcification bone slices, and the bone morphometry bone mass parameters and surface parameters were measured. The parameters of each group were analyzed by variance. Results1 The bone mass parameters in the pathological group were smaller than those in the normal group (P < 0.05). The surface parameters showed that the parameters of osteogenesis decreased, the parameters of osteotomy increased, and the bone turnover was accelerated (P <0. 5 ). 2 The bone mass parameters of each Chinese medicine group were higher than those of the pathological group (P < 0.05); the surface parameters were reversed. At the same time, some osteoblasts are seen to be particularly active, and the lower bone mass is higher than the surrounding static surface. 3 The thickness of cortical bone and bone mineralization of kidney and spleen + calcium group were higher than those of other Chinese medicine and calcium groups (P < 0.05). It is suggested that the negative balance and bone turnover of reconstruction are the histological mechanisms leading to bone loss after testis in male rats. The simultaneous application of kidney, spleen, and calcium supplementation can inhibit bone resorption, promote bone formation, reduce bone turnover, increase body absorption and utilization of calcium, and thus affect the process of bone mineralization. It is the most effective treatment of osteoporosis in elderly men. Good method