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白芍为常用中药,具有平肝止痛、养血调经、敛阴止汗之功效,其主要成分为芍药甙。本文采用高效液相法对白芍的不同炮制品中芍药甙的含量进行了测定,结果报道如下。一、材料及仪器材料白芍购于金华医药站,经杨建国鉴定为毛茛科植物白芍Paeonia lactiflora Pall.的干燥根。6种炮制品均按《中国药典》1990版规定自制。甲醇(色谱纯)。超净水为用新鲜蒸馏水自制。芍药甙对照品(中国药品生物制品检定所,批号736—8701)。仪器美国Waters 510泵,U6K手动进样器,490紫外检测器,745积分仪。
White peony is a commonly used traditional Chinese medicine. It has the effects of calming liver pain, nourishing blood, regulating menstruation, and accumulating yin and antiperspirant. Its main ingredient is peony. In this paper, the content of paeoniflorin in different processed products of Radix Paeoniae Alba was determined by HPLC, and the results were reported as follows. 1. Materials and Instrument Materials White peony was purchased from Jinhua Medical Station and identified by Yang Jianguo as the dried root of the Paeonia lactiflora Pall. All six kinds of processed products were made according to the “Chinese Pharmacopoeia” 1990 edition. Methanol (chromatographically pure). Ultra clean water is made with fresh distilled water. Pharmacopeia reference drug (China National Institute for the Control of Pharmaceutical and Biological Products, lot 736-8701). Instrument US Waters 510 pump, U6K manual sampler, 490 UV detector, 745 integrator.